Incubation:Article Title: A simple, highly efficient Agrobacterium tumefaciens ‐mediated moss transformation system with broad applications
Article Snippet: .. A single A. tumefaciens colony from a fresh plate was inoculated into 5 mL liquid LB medium supplemented with 50 mg/L kanamycin and 25 mg/L rifampicin (Solarbio, China) and incubated with shaking (220 rpm) at 28 °C for 20–24 h. To increase the number of Agrobacterium cells for the second activation, 1 mL of the bacterial suspension was inoculated into 50 mL of LB liquid medium supplemented with the appropriate antibiotics and incubated with shaking (220 rpm) at 28 °C for 12–16 h. The Agrobacterium cells were collected by centrifugation, resuspended in 50 mL AB:MES salts (17.2 mM K 2 HPO 4 , 8.3 mM NaH 2 PO 4 , 18.7 mM NH 4 Cl, 2 mM KCl, 1.25 mM MgSO 4 , 100 μM CaCl 2 , 10 μM FeSO 4 , 50 mM MES, 2% glucose [w/v], and 200 μM acetosyringone, pH 5.5), and grown overnight. ..
Article Title: Influence of 5’-UTR base types on translation efficiency in Escherichia coli
Article Snippet: .. After 89 incubation at 37°C for 1 h, the bacterial solutions in the centrifuge tubes were centrifuged at 90 4000×g for 2 min, 40 μL of suspension was taken to 4 mL fresh liquid LB medium containing 91 ampicillin and cultured at 37°C, 250 rpm for 12 h. The next day, the overnight cultures of the four 92 libraries were transferred to fresh liquid LB medium containing ampicillin and IPTG at a ratio of 93 1:100 and cultured at 37°C, 250 rpm for 5.5 h. Before the flow cytometry analysis, cells were 94 centrifuged (4000×g for 2 min), washed twice in 0.01M ice-cold PBS buffer (Solarbio), 95 resuspended in ice-cold PBS buffer. .. Cells were analyzed using the flow cytometer (BD FACS 96 Aria III, Becton Dickinson) which monitors the sfGFP fluorescence intensities at 488 nm/507 nm.
Activation Assay:Article Title: A simple, highly efficient Agrobacterium tumefaciens ‐mediated moss transformation system with broad applications
Article Snippet: .. A single A. tumefaciens colony from a fresh plate was inoculated into 5 mL liquid LB medium supplemented with 50 mg/L kanamycin and 25 mg/L rifampicin (Solarbio, China) and incubated with shaking (220 rpm) at 28 °C for 20–24 h. To increase the number of Agrobacterium cells for the second activation, 1 mL of the bacterial suspension was inoculated into 50 mL of LB liquid medium supplemented with the appropriate antibiotics and incubated with shaking (220 rpm) at 28 °C for 12–16 h. The Agrobacterium cells were collected by centrifugation, resuspended in 50 mL AB:MES salts (17.2 mM K 2 HPO 4 , 8.3 mM NaH 2 PO 4 , 18.7 mM NH 4 Cl, 2 mM KCl, 1.25 mM MgSO 4 , 100 μM CaCl 2 , 10 μM FeSO 4 , 50 mM MES, 2% glucose [w/v], and 200 μM acetosyringone, pH 5.5), and grown overnight. ..
Suspension:Article Title: A simple, highly efficient Agrobacterium tumefaciens ‐mediated moss transformation system with broad applications
Article Snippet: .. A single A. tumefaciens colony from a fresh plate was inoculated into 5 mL liquid LB medium supplemented with 50 mg/L kanamycin and 25 mg/L rifampicin (Solarbio, China) and incubated with shaking (220 rpm) at 28 °C for 20–24 h. To increase the number of Agrobacterium cells for the second activation, 1 mL of the bacterial suspension was inoculated into 50 mL of LB liquid medium supplemented with the appropriate antibiotics and incubated with shaking (220 rpm) at 28 °C for 12–16 h. The Agrobacterium cells were collected by centrifugation, resuspended in 50 mL AB:MES salts (17.2 mM K 2 HPO 4 , 8.3 mM NaH 2 PO 4 , 18.7 mM NH 4 Cl, 2 mM KCl, 1.25 mM MgSO 4 , 100 μM CaCl 2 , 10 μM FeSO 4 , 50 mM MES, 2% glucose [w/v], and 200 μM acetosyringone, pH 5.5), and grown overnight. ..
Article Title: Influence of 5’-UTR base types on translation efficiency in Escherichia coli
Article Snippet: .. After 89 incubation at 37°C for 1 h, the bacterial solutions in the centrifuge tubes were centrifuged at 90 4000×g for 2 min, 40 μL of suspension was taken to 4 mL fresh liquid LB medium containing 91 ampicillin and cultured at 37°C, 250 rpm for 12 h. The next day, the overnight cultures of the four 92 libraries were transferred to fresh liquid LB medium containing ampicillin and IPTG at a ratio of 93 1:100 and cultured at 37°C, 250 rpm for 5.5 h. Before the flow cytometry analysis, cells were 94 centrifuged (4000×g for 2 min), washed twice in 0.01M ice-cold PBS buffer (Solarbio), 95 resuspended in ice-cold PBS buffer. .. Cells were analyzed using the flow cytometer (BD FACS 96 Aria III, Becton Dickinson) which monitors the sfGFP fluorescence intensities at 488 nm/507 nm.
Centrifugation:Article Title: A simple, highly efficient Agrobacterium tumefaciens ‐mediated moss transformation system with broad applications
Article Snippet: .. A single A. tumefaciens colony from a fresh plate was inoculated into 5 mL liquid LB medium supplemented with 50 mg/L kanamycin and 25 mg/L rifampicin (Solarbio, China) and incubated with shaking (220 rpm) at 28 °C for 20–24 h. To increase the number of Agrobacterium cells for the second activation, 1 mL of the bacterial suspension was inoculated into 50 mL of LB liquid medium supplemented with the appropriate antibiotics and incubated with shaking (220 rpm) at 28 °C for 12–16 h. The Agrobacterium cells were collected by centrifugation, resuspended in 50 mL AB:MES salts (17.2 mM K 2 HPO 4 , 8.3 mM NaH 2 PO 4 , 18.7 mM NH 4 Cl, 2 mM KCl, 1.25 mM MgSO 4 , 100 μM CaCl 2 , 10 μM FeSO 4 , 50 mM MES, 2% glucose [w/v], and 200 μM acetosyringone, pH 5.5), and grown overnight. ..
Control:Article Title: Community Structure, Growth-Promoting Potential, and Genomic Analysis of Seed-Endophytic Bacteria in Stipagrostis pennata
Article Snippet: .. In the osmotic stress tolerance test, liquid LB medium containing 10%, 20%, 30%, and 40% PEG6000 (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China, Cat: P8250, CAS: 25322-68-3, MW 5400–6600 Da) was prepared, with medium without PEG6000 used as the control. ..
Cell Culture:Article Title: Influence of 5’-UTR base types on translation efficiency in Escherichia coli
Article Snippet: .. After 89 incubation at 37°C for 1 h, the bacterial solutions in the centrifuge tubes were centrifuged at 90 4000×g for 2 min, 40 μL of suspension was taken to 4 mL fresh liquid LB medium containing 91 ampicillin and cultured at 37°C, 250 rpm for 12 h. The next day, the overnight cultures of the four 92 libraries were transferred to fresh liquid LB medium containing ampicillin and IPTG at a ratio of 93 1:100 and cultured at 37°C, 250 rpm for 5.5 h. Before the flow cytometry analysis, cells were 94 centrifuged (4000×g for 2 min), washed twice in 0.01M ice-cold PBS buffer (Solarbio), 95 resuspended in ice-cold PBS buffer. .. Cells were analyzed using the flow cytometer (BD FACS 96 Aria III, Becton Dickinson) which monitors the sfGFP fluorescence intensities at 488 nm/507 nm.
Flow Cytometry:Article Title: Influence of 5’-UTR base types on translation efficiency in Escherichia coli
Article Snippet: .. After 89 incubation at 37°C for 1 h, the bacterial solutions in the centrifuge tubes were centrifuged at 90 4000×g for 2 min, 40 μL of suspension was taken to 4 mL fresh liquid LB medium containing 91 ampicillin and cultured at 37°C, 250 rpm for 12 h. The next day, the overnight cultures of the four 92 libraries were transferred to fresh liquid LB medium containing ampicillin and IPTG at a ratio of 93 1:100 and cultured at 37°C, 250 rpm for 5.5 h. Before the flow cytometry analysis, cells were 94 centrifuged (4000×g for 2 min), washed twice in 0.01M ice-cold PBS buffer (Solarbio), 95 resuspended in ice-cold PBS buffer. .. Cells were analyzed using the flow cytometer (BD FACS 96 Aria III, Becton Dickinson) which monitors the sfGFP fluorescence intensities at 488 nm/507 nm.
MTT Assay:Article Title: Polydopamine and Magnesium Ions Loaded 3D‐Printed Ti‐6Al‐4V Implants Coating with Enhanced Osteogenesis and Antibacterial Abilities
Article Snippet: Human bone tissue has a specific selfrepair ability after being damaged, whereas, this ability is limited in some exceptional cases.. Therefore, necessary medical treatments such as implants and scaffolds are required to repair and replace the bone tissue.. Titanium and its alloy (Ti-6Al-4V) have been widely used in implantation because of their great general properties.
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